| Brucellosis, a reemerging zoonotic disease caused by the Gram-negative coccobacillus Brucella, poses diagnostic
challenges due to nonspecific clinical manifestations. This study aimed to develop an indirect ELISA utilizing smooth
lipopolysaccharide antigens from Brucella abortus 99 for anti-Brucella antibody detection. Serum samples from 1304
asymptomatic individuals were screened using the plate agglutination test (RBPT) and standard tube agglutination test
(STAT), followed by indirect ELISA. Compared to RBPT and STAT, indirect ELISA demonstrated higher sensitivity,
detecting 20 positive samples, highlighting its potential for improved brucellosis diagnosisddd |
